2xYT Medium

Features

Quantity: 500g
Appearance: Beige powder. Autoclaved medium should be amber, slightly opalescent.
Storage: 2ºC – 25ºC. When not in use, keep container closed to avoid hydration.

Formulation (g/L)
Tryptone: 16,00
Yeast Extract: 10,00
NaCl: 5,00
Final pH (25ºC): 7,0 ± 0,2

Preparation:
Add 31g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121ºC for 15 to 20 minutes. Store at 2ºC to 8ºC.

Supplements
2xYT medium (2xTY medium) is a rich growth medium, optimized for growth and maintenance of filamentous phage such as M13. It contains all the nutritional requirements for E.coli. Tryptone and Yeast Extract are the sources for carbon, nitrogen, vitamins, minerals, and amino acids essential for growth, as well as growth factors that allow phages to reproduce without weakening the host cells. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Many supplements, including antibiotics, are heat-sensitive and cannot be autoclaved. These should be filter-sterilized and added to the medium after it has cooled down.