Description
The GRS Genomic DNA Kit – Tissue – provides an efficient and fast method for the purification and or concentration of high quality total DNA (including genomic DNA, mitochondrial DNA and viral DNA) from a variety of tissues, including tailsnips, liver, kidney, brain, adipose tissue, earpunches, insects, and FFPE. Eluted purified DNA (approximately 20-30kb) is suitable for PCR, and other enzymatic reactions.
Principle
The GRS Genomic DNA Kit – Tissue – uses chaotropic salts and proteinase K to lyse cells and to denature proteins. The provided micropestle improves tissue sample homogenization and therefore to reduce the time required for cell lysis. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column (1). Contaminants such as proteins, divalent cations, unincorporated nucleotides, and enzyme inhibitors are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified genomic DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed within an hour without phenol/chloroform extraction or alcohol precipitation, with typical DNA yields of up to 50μg.