Description
Product: Dehydrated powder for the preparation of nutritionally rich solid medium (plates) for the growth and maintenance of recombinant strains of Escherichia
coli in molecular biology studies.
Quantity: 500g
Appearance: Beige powder. Autoclaved medium should be amber, slightly opalescent
Storage: 2ºC – 25ºC. When not in use, keep container closed to avoid hydration.
Preparation:
Add 35g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers
and sterilize by autoclaving at 121ºC for 15 to 20 minutes. Cool to 45-50ºC and dispense into plates. Plates should be stored at 8ºC to 15ºC.
Supplements
LB Agar (Lennox) is a rich growth medium, which contains all the nutritional requirements for E.coli. Tryptone and Yeast Extract are the sources for carbon, nitrogen, vitamins, minerals, and amino acids
essential for growth, whereas sodium chloride supplies essential electrolytes for transport and osmotic balance. For faster growth, medium can be supplemented with glucose (0.1%) or glycerol (0.4%).
Many supplements, including antibiotics, are heat-sensitive and cannot be autoclaved. These should be filter-sterilized and added to the medium after it has cooled down and prior to solidification.
Formulation (g/L)
Tryptone: 10,00
Yeast Extract: 5,00
NaCl: 5,00
Bacteriological Agar: 15,00
Final pH (25ºC): 7,0 ± 0,2