Description
Xpert Fast Probe (uni) Blue 2X mastermix contains all components, except primers and template, for the amplification and detection of DNA in qPCR based on a wide range of probe-based technologies, including Taqman®, Molecular Beacons® and Scorpion probes®. It consists of the combination of a highly efficient enzyme with a novel low inhibitory technology, which prevents the formation of primer-dimers, thus allowing for extremely high sensitivity and specificity. Xpert Fast Probe (uni) Blue can be used to quantify virtually any DNA target, including extremely low-copy number targets, with minimal effort and optimization. Xpert Fast Probe (uni) Blue 2X mastermix is supplied with a separate vial of ROX reference dye, so it can be used with most*) real-time PCR instruments.
This Mastermix includes an inert dye (Blue) as visual aid for pipetting. The dye does not inhibit qPCR but may impact the intensity of some fluorescent probes.
Features
Properties:
– Early Ct values – Rapid extenstion rate
– High Efficiency in multiplex reactions
– High Efficiency in GC or AT-rich templates
– Extreme sensitivity – increased limit of detection
– Allows for standard and fast cycling
– Compatible with most qPCR machines (see FAQs)
This Mastermix includes an inert dye (Blue) as visual aid for pipetting. The dye does not inhibit qPCR but may impact the intensity of some fluorescent probes.
Applications:
– Absolute quantification
– Gene expression analysis
– Diagnostic real-time PCR
– Low-copy number target genes
– Multiplex or singleplex
Components:
Xpert Fast Probe is supplied in multiples of 1mL, for your convenience!
Xpert Fast Probe is supplied as a convenient 2x mastermix containing all required components except specific primers. 1mL is enough for 100 reactions of 20μl. The mastermix is supplied with a separate vial of 100X (50µM) ROX solution. Depending on the PCR equipment (see FAQs), prior to use, one should add 2µl of ROX (LowROX (final concentation of 50nM), 20µl (HighROX (final concentation of 500nM)), or nothing (No ROX). Once ROX has been added, the mastermix might be used immediately or stored at -20ºC.
Storage:
-20ºC for at least 1 year
