Cloning

  • Ampicillin (sodium salt) Quick View
    • Ampicillin (sodium salt) Quick View
    • Ampicillin (sodium salt)

    • Ampicillin is a bactericidal agent that acts by inhibition of transpeptidase, which is required for cell wall synthesis, and as such functions as a broad-spectrum antibiotic, effective against Gram(+) and Gram(-) bacteria.    
  • Carbenicillin (disodium salt) Quick View
    • Carbenicillin (disodium salt) Quick View
    • Carbenicillin (disodium salt)

    • Carbenicillin, like ampicillin, is an antibiotic of the ß-lactam class and acts in an identical way as ampicillin, with the advantage that it is more stable. It is a bactericidal agent that acts by inhibition of transpeptidase, which is required for    
  • Chloramphenicol Quick View
    • Chloramphenicol Quick View
    • Chloramphenicol

    • Chloramphenicol is a bacteriostatic agent that acts by binding to the 50S ribosomal subunit inhibiting peptidyl transferase required for protein synthesis. Chloramphenicol is broad-spectrum antibiotic, effective against a wide variety of Gram(+) and Gram(-) bacteria.    
  • Gentamicin (sulphate) Quick View
    • Gentamicin (sulphate) Quick View
    • Gentamicin (sulphate)

    • Gentamicin is an aminoglycoside antibiotic complex comprised of 3 major components (C1, C1a, and C2). It inhibits protein synthesis by binding to the 30S subunit of the ribosome. It is a broad-spectrum antbiotic with activity against Gram (+) and Gram (-) bacteria, and mycoplasmas.    
  • IPTG (max 5ppm dioxane) Quick View
    • IPTG (max 5ppm dioxane) Quick View
    • IPTG (max 5ppm dioxane)

    • IPTG (Isopropyl ß-D-thiogalactopyranoside) is a lactose analog that mimics allolactose, which triggers transcription of the lac operon by inactivation of the lac repressor. As such, IPTG can be used to induce protein expression of heterologous proteins that are encoded by genes under the control of the lac operator.  
  • Kanamycin (sulphate) Quick View
    • Kanamycin (sulphate) Quick View
    • Kanamycin (sulphate)

    • Kanamycin, from Streptomyces kanamyceticus, is a bactericidal agent that acts by binding to the 30S subunit inhibiting protein synthesis, and as such functions as a broad-spectrum antibiotic, effective against many Gram(+) and most Gram(-) bacteria.    
  • T4 DNA Ligase (5U/uL) Quick View
    • T4 DNA Ligase (5U/uL) Quick View
    • T4 DNA Ligase (5U/uL)

    • T4 DNA Ligase for the ligation of complementary cohesive ends or blunt ends, in restriction-ligation cloning of DNA fragments into plasmids.    
  • Tetracycline (hydrochloride) Quick View
    • Tetracycline (hydrochloride) Quick View
    • Tetracycline (hydrochloride)

    • Tetracycline is a bacteriostatic agent that acts by preventing aminoacyl tRNA from binding to the ribosome A site, thus inhibiting protein synthesis. It also binds to the bacterial 50S ribosomal subunit, causing leaking of intracellular components via the membrane. Tetracycline is broad-spectrum antibiotic, effective against Gram(+) and Gram(-) bacteria.    
  • X-Gal Quick View
    • X-Gal Quick View
    • X-Gal

    • X-gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) is a synthetic analog of lactose that mimics lactose as an inert chromogenic substrate for ß-galactosidase.    
  • Xpert TA Easy Cloning Kit Quick View
    • Xpert TA Easy Cloning Kit Quick View
    • Xpert TA Easy Cloning Kit

    • Xpert TA Easy cloning kit for direct ligation of PCR fragments containing 3´-A overhangs (generated using non-proofreading DNA polymerases such as Taq or blends thereof) into a ready-to-use stable linearized vector.