Antibiotic-Antimycotic Solution is a mixture of Penicillin, Streptomycin, and Amphotericin B (see: formulation) in saline solution, and is used to prevent bacterial and fungal contamination in cell cultures. The effective combined action of the antibiotics Penicillin and Streptomycin result in toxicity towards gram-negative and gram-positive bacteria, whereas Amphotericin B acts against yeast and multicellular fungi. This product is provided as a ready-to-use 100x concentrate.
Penicillin: 10000 Units/ml
Streptomycin: 10.00 mg/ml
Amphotericin B: 25.00 μg/ml
Quantity: 100 ml
Appearance: yellowish solution
Endotoxin: ≤ 0.5EU/ml
Storage: -20ºC. Once defrosted, store at +2?C-8?C for up to 4 weeks. If needed, one can freeze the solution again, promptly after using. For this, aseptically prepare aliquots of convenient volume, as repeated freeze/thaw cycles should be avoided.
This product is provided as a ready-to-use 100x concentrate and the recommended final concentration is 10ml/l of cell culture medium.