Carbenicillin, like ampicillin, is an antibiotic of the ß-lactam class and acts in an identical way as ampicillin, with the advantage that it is more stable. It is a bactericidal agent that acts by inhibition of transpeptidase, which is required for cell wall synthesis, and as such functions as a broad-spectrum antibiotic, effective against Gram(+) and Gram(-) bacteria. This semi-synthetic derivate from Penicillin, like Penicillin, can be inactivated by ß-lactamase, which hydrolyzes the ß-lactam ring. Therefore, carbenicillin is frequently used for the selection of bacteria transformed with a vector harbouring the gene encoding ß-lactamase (bla), which turns them resistant, from untransformed cells.
Appearance: White, slightly beige powder.
Storage: 2ºC – 8ºC for at least 1 years, at -20ºC at least 3 years
Preparation: Prepare a stock solution of 4-50mg/ml in ultrapure water or 50% ethanol, and filter sterilize. Do not autoclave. Store stock solution at +4ºC for several weeks or at -20ºC for up to 6 months.
Usage: Carbenicillin is used in the same way as ampicillin. In comparison with ampicillin, Carbenicillin is more stable. In culture media, it is more resistant to heat and low-pH induced degradation over time, making it a very useful antibiotic for large-scale liquid culture growth. Moreover, growth of satellite colonies on agar plates is less likely to occur. For incorporation in agar plates, add carbenicillin to a final concentration of 20-100μg/ml (depending on, among other factors, type of vector (low-copy vs high-copy number plasmid)) by adding to autoclaved media agar, just prior to pouring the plates, or to broth after cooling down to room temperature.
MW: 422,4 g/mol
Purity: >90% (on dry basis)
Solubility (H2O): 50mg/ml