Chloramphenicol is a bacteriostatic agent that acts by binding to the 50S ribosomal subunit inhibiting peptidyl transferase required for protein synthesis.
Chloramphenicol is broad-spectrum antibiotic, effective against a wide variety of Gram(+) and Gram(-) bacteria. Chloramphenicol is frequently used for the selection of bacteria transformed with a vector harbouring a gene conferring chloramphenicol resistance (CAT), but it is also used to increase production of low-copy number plasmids, containing the pMB1 origin.
Appearance: white or slightly yellow needle crystals or crystalline solid
Storage: 2ºC–8ºC for at least to 3 years.
Preparation: Prepare a stock solution of 25-34mg/ml in 100% ethanol. Do not autoclave. Store stock solution at -20ºC.
Usage: For incorporation in agar plates or broth, add chloramphenicol to a final concentration of 10-170μg/ml by adding to autoclaved media agar, just prior to pouring the plates, or to broth after cooling down to room temperature. Suggested protocol for increasing production of low-copy number plasmids: grow cells in rich medium, containing appropriate antibiotic, until OD600nm = 0,4 and then add chloramphenicol to a final density of 170μg/ml. Incubate with vigorous shaking for another 12-16h. Depending on medium composition, cell line, plasmid, temperature and other conditions, optimize initial density, incubation time and/or final chloramphenicol concentration.
MW: 323,1 g/mol
Purity: >98% (on dry basis)
Solubility (H2O): 2,5g/l
Water content: <0,5%