Gentamicin is an aminoglycoside antibiotic complex comprised of 3 major components (C1, C1a, and C2). It inhibits protein synthesis by binding to the 30S subunit of the ribosome. It is a broad-spectrum antbiotic with activity against Gram (+) and Gram (-) bacteria, and mycoplasmas.
Appearance: white or slightly yellow needle crystals or crystalline solid
Storage: 2ºC–8ºC for at least to 3 years.
Preparation: Prepare a stock solution of 50mg/ml in ultrapure water, and filter sterilize. Do not autoclave. Store stock solution at 2ºC-8ºC.
Usage: For incorporation in agar plates or broth, add chloramphenicol to a final concentration of 50μg/ml by adding to autoclaved media agar, just prior to pouring the plates, or to broth after cooling down to room temperature.
Gentamycin C1: 25 ? 50%
Gentamycin C1a: 10 ? 35%
Gentamycin C2: 25 ? 55%
Gentamycin C1: C21H43N5O7 (Mw=477,6g/mol)
Gentamycin C1a: C19H39N5O7 (Mw=449,5g/mol)
Gentamycin C2: C20H41N5O7 (Mw=463,6g/mol)
Potency: >590μg/mg (on dry basis)
Water content: <18%
Specific optical rotation: +107? to +121?
Solubility (H2O): 50mg/ml