Description
The GRS circulating cell-free DNA/RNA Purification Kit includes a specially developed removable extension tube that, when inserted in the mini spin column, allows for the expansion of the starting volume up to 5 ml. Antibodies and other proteins are eliminated by Proteinase K digestion, and the digest is mixed with a unique Binding Buffer that is optimized to allow easy binding of DNA and RNA to the glass fiber matrix of the spin column. The mixture can be passed through the column either by centrifugation or by using a vacuum manifold system. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified DNA/RNA is subsequently eluted with RNase-free water. The entire procedure can be completed in approximately 60 minutes