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GRS circulating cell-free DNA/RNA Purification Kit

The GRS circulating cell-free DNA/RNA Purification Kit provides an efficient and fast method for the isolation of high-quality DNA and RNA from up to 5 ml of serum or plasma. The purified DNA/RNA is suitable for a wide variety of downstream applications including qPCR and DNA sequencing.
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Description

The GRS circulating cell-free DNA/RNA Purification Kit includes a specially developed removable extension tube that, when inserted in the mini spin column, allows for the expansion of the starting volume up to 5 ml. Antibodies and other proteins are eliminated by Proteinase K digestion, and the digest is mixed with a unique Binding Buffer that is optimized to allow easy binding of DNA and RNA to the glass fiber matrix of the spin column. The mixture can be passed through the column either by centrifugation or by using a vacuum manifold system. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified DNA/RNA is subsequently eluted with RNase-free water. The entire procedure can be completed in approximately 60 minutes

Additional information

Quantidade

50 preps

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Product Info GRS circulating cell-free DNA/RNA Purification Kit

MSDS – GRS circulating cell-free DNA/RNA Purification Kit

Order Information

Reference Description Quantity
GK20.0020 GRS circulating cell-free DNA/RNA Purification Kit 50 preps

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Product: GRS circulating cell-free DNA/RNA Purification Kit

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FAQs

How to use GRS circulating cell-free DNA/RNA Purification Kit:
Quality Control
The quality of the TripleXtractor directRNA kit is tested on a lot-to-lot basis by extracting RNA from a 1 ml Escherichia coli culture with an OD ~1.3 (~109 cells). Purified RNA is analyzed by spectrophotometry and by agarose gel electrophoresis.