Description
GRS DNA Loading Buffer Green (6X) is a pre-mixed Loading Buffer for Agarose and Polyacrylamide gel electrophoresis, containing Xylene Cyanol FF and Orange G as tracking dyes. EDTA is included to chelate Magnesium (up to 10 mM), stopping enzymatic reactions.
Contents
Solution of 10mM Tris-HCl pH 7,6; 60mM EDTA; 0,03% Bromophenol Blue; 0.15% Orange G; 60% Glycerol.
Applications
Preparation of DNA samples and markers for loading onto agarose and polyacrylamide gels. This Loading Buffer is a 6X concentrate: add 1μl of GRS DNA Loading Buffer Green (6x) per 5 μl of DNA sample.
Dye Migration
Migration of the tracking dyes depend on the type of gel, concentration of the gel and the electrophoresis buffer. For example, on a 1.5% agarose gel the tracking dyes co-migrate with DNA fragments of the following sizes:
Xylene Cyanol FF
TAE: ~ 2.8 kb
TBE: ~ 1.8 kb
VOLTage Fast: ~ 900bp
Orange G
TAE: ~ 50bp
TBE: ~ 50bp
VOLTage Fast: ~ <40bp
Specifications
Free of DNases and RNases.
Quality Control
Absence of endodeoxyribonucleases, exodeoxyribonucleases, and ribonucleases was confirmed by appropriate assays.
Storage
Store at room temperature or +4ºC for up to at least 12 months. For longer storage, store at -20ºC.