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GRS Hotstart DNA Polymerase

GRS Hotstart Taq DNA polymerase consists of GRS Taq bound to a specific antibody that blocks polymerase activity, preventing or minimizing the synthesis of non-specific products and primer-dimers prior to PCR cycling, which results in an overall higher specificity.

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Description

Main Application: Hotstart Routine PCR

GRS Hotstart Taq DNA polymerase consists of GRS Taq bound to a specific antibody that blocks polymerase activity, preventing or minimizing the synthesis of non-specific products and primer-dimers prior to PCR cycling, which results in an overall higher specificity. The heat-labile antibody can be rapidly inactivated by increasing the temperature for a few minutes to 95ºC (hot-start) resulting in polymerase activation.

Like GRS Taq, this enzyme has 5´→3´ polymerase activity but no 3´→5´ exonuclease activity (no proofreading). It is suitable for amplifying DNA targets up to 5kb and produces 3´ A-overhangs allowing TA-cloning

Additional information

Quantidade

(+dNTPs) 2500 U, (+dNTPs) 500 U, 1.25mL, 2500 U, 500 U, 5x 1.25mL

Features

Properties:
Amplicon size:  up to 5kb
Extension Rate 1kb/min
Hotstart:            Yes
A-overhang:      Yes

Applications:
– Hotstart Routine PCR

Components (kit)
GRS Hotstart Taq Polymerase is supplied with PCR Buffer (10x concentrated), consisting of 100mM Tris (pH 8.9), 500mM KCl, supplemented with BSA (2.5mg/ml) and with a separate vial of 25mM MgCl2.

Product GE71.0500 GE71.2500 GE72.0500 GE72.2500
GRS Hotstart  Taq (5U/µl) 100µl 5x 100µl 100µl 5x 100µl
PCR Buffer (10X) 1,5ml 5x 1,5ml 1,5ml 5x 1,5ml
MgCl2 (25mM) 1,5ml 5x 1,5ml 1,5ml 5x 1,5ml
dNTPs (10mM) 1,0ml 3x 1,0ml

Storage:
-20ºC for at least 1 year.

Components (mastermix)
GRS Hotstart Taq DNA Polymerase is supplied as a convenient 2x mastermix containing all required components, except specific primers. One vial of 1,25 ml is sufficient for 100 reactions of 25μl (or 125 reactions of 20μl). Final concentration of MgCl2 will be 3mM.

Product GE73.0100 GE73.5100
GRS Hotstart  2X Mastermix 1,25ml 5x 1,25ml

Storage:
-20ºC for at least 1 year.

Documents

Product Info GRS Hotstart Mastermix

Product Info GRS Hotstart DNA Polymerase

Order Information

 

Reference Description Quantity
GE71.0500 GRS Taq DNA Polymerase 500 U
GE71.2500 GRS Taq DNA Polymerase 2500 U
GE72.0500 GRS Taq DNA Polymerase (+dNTPs) 500 U
GE72.2500 GRS Taq DNA Polymerase (+dNTPs) 2500 U
GE73.0100 GRS Taq 2X Mastermix (100rxn 25uL) (100rxn 25uL) 1.25 mL
GE73.5100 GRS Taq 2X Mastermix (500rxn 25uL) (500rxn 25uL) 5x 1.25 mL

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FAQs

How to use GRS Hotstart DNA Polymerase:
Functionally tested in PCR. Bacterial DNA free (tested by PCR). Absence of endonucleases, exonucleases, and ribonucleases was confirmed by appropriate assays.