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GRS Taq DNA Polymerase

For Routine PCR GRS Taq DNA polymerase is a recombinant thermostable enzyme (94kDa monomer) with identical characteristics as native Taq regarding activity, specificity, thermostability, and performance in PCR.

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Description

Main Application: Routine PCR

GRS Taq DNA polymerase is a recombinant thermostable enzyme (94kDa monomer) with identical characteristics as native Taq regarding activity, specificity, thermostability, and performance in PCR.

GRS Taq possesses 5´→3´ polymerase activity as well as weak 5´→3´ exonuclease activity. The enzyme has no 3´→5´ exonuclease activity (thus no proofreading). GRS Taq is suitable for amplification of targets up to 5kb and is capable of incorporating modified nucleotides (e.g. fluorescent labeled nucleotides). GRS Taq produces 3´ A-overhangs allowing TA-cloning.

Additional information

Quantidade

(+dNTPs) 500 U, 1.25mL, 2500 U, 2500U (+dNTPs), 500 U, 5x 1.25mL

Features

Properties:
Amplicon size:  up to 5kb
Extension Rate 1kb/min
Hotstart:            No
A-overhang:      Yes

Applications:
– Routine PCR

Components (kit)
Supplied with PCR Buffer (10x concentrated), consisting of 500mM Tris-HCl (pH 9.4), 1% Tween®20 and 150mM ammonium sulphate, and with separate 25mM MgCl2.

Product GE01.0500 GE01.2500 GE11.0500 GE11.2500 GE11.5000
GRS Taq (5U/µl) 100µl 5x 100µl 100µl 5x 100µl 10x 100µl
PCR Buffer (10X) 1,5ml 5x 1,5ml 1,5ml 5x 1,5ml 10x 1,5ml
MgCl2 (25mM) 1,5ml 5x 1,5ml 1,5ml 5x 1,5ml 10x 1,5ml
dNTPs (10mM) 1,0ml 3x 1,0ml 6x 1,0ml

Storage:
-20ºC for at least 1 year.

Components (mastermix)
GRS Taq DNA polymerase is supplied as a convenient 2x mastermix containing all required components except specific primers. 1.25mL is enough for 100 reactions of 25μl (or 125 reactions of 20μl). Final concentration of MgCl2 will be 2mM.

Product GE02.0100 GE02.5100
GRS Taq 2X Mastermix 1,25ml 5x 1,25ml

Storage:
-20ºC for at least 1 year.

Documents

Product Info – GRS Taq 2x Mastermix

Product Info – GRS Taq DNA Polymerase

Order Information

Reference Description Quantity
GE01.0500 GRS Taq DNA Polymerase 500 U
GE01.2500 GRS Taq DNA Polymerase 2500 U
GE11.0500 GRS Taq DNA Polymerase (+dNTPs) 500 U
GE11.2500 GRS Taq DNA Polymerase (+dNTPs) 2500 U
GE02.0100 GRS Taq 2X Mastermix (100rxn 25uL) (100rxn 25uL) 1.25 mL
GE02.5100 GRS Taq 2X Mastermix (500rxn 25uL) (500rxn 25uL) 5x 1.25 mL

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Product: GRS Taq DNA Polymerase

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FAQs

How to use GRS Taq DNA Polymerase:
Functionally tested in PCR. Bacterial DNA free (tested by PCR). Absence of endonucleases, exonucleases, and ribonucleases was confirmed by appropriate assays.