The GRS Plasmid Purification Kit provides an efficient and fast method for the purification of highquality plasmid DNA from 1-6 ml of cultured bacterial cells. Eluted DNA is suitable for all common downstream applications including PCR, enzymatic restriction digestion, cloning and DNA sequencing.
The GRS Plasmid Purification Kit is based on a modified Alkaline Lysis method used to obtain minimal genomic DNA contaminants (1). RNAse A treatment reduces contamination with RNA. Blue Lysis Buffer is added to ensure efficient cell lysis and SDS precipitation. The buffer system is optimized to allow binding of plasmid DNA in the presence of chaotropic salts to the glass fiber matrix of the spin column (2). Contaminants are removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified plasmid DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed in within 20 minutes without phenol/chloroform extraction or alcohol precipitation, with a typical DNA yields of 20-30μg for high-copy number plasmids or 3-10μg for low-copy number plasmids from 4,5 ml of cultured bacterial cells.