GRiSP

GRS Plasmid Purification Kit - Mini

The GRS Plasmid Purification Kit provides an efficient and fast method for the purification of high quality plasmid DNA from 1-6 ml of cultured bacterial cells ( miniprep ). Eluted DNA is free of RNA and suitable for all common downstream application
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Description

The GRS Plasmid Purification Kit provides an efficient and fast method for the purification of highquality plasmid DNA from 1-6 ml of  cultured bacterial cells. Eluted DNA is suitable for all common downstream applications including PCR, enzymatic restriction digestion, cloning and DNA sequencing.

Principle
The GRS Plasmid Purification Kit is based on a modified Alkaline Lysis method used to obtain minimal genomic DNA contaminants (1). RNAse A treatment reduces contamination with RNA. Blue Lysis Buffer is added to ensure efficient cell lysis and SDS precipitation. The buffer system is optimized to allow binding of plasmid DNA in the presence of chaotropic salts to the glass fiber matrix of the spin column (2). Contaminants are removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified plasmid DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed in within 20 minutes without phenol/chloroform extraction or alcohol precipitation, with a typical DNA yields of 20-30μg for high-copy number plasmids or 3-10μg for low-copy number plasmids from 4,5 ml of cultured bacterial cells.

Additional information

Quantidade

100preps/kit, 3x100preps/kit, 5x100preps/kit

Features

Sample : 1,5 ml of cultured bacterial cells (up to 6 ml)
Expected Yield : up to 50 μg of plasmid DNA
Format : spin column
Operation Time : within 20 minutes
Elution Volume : 30-100 μl

Documents

Download Product Info Datasheet

Download MSDS GK13 GRS Plasmid Purification Kit – Mini v7E0502

 

Order Information

Reference Description Quantity
GK13.0100 GRS Plasmid Purification Kit-Mini 100preps/kit
GK13.3100 GRS Plasmid Purification Kit-Mini 3x100preps/kit
GK13.5100 GRS Plasmid Purification Kit-Mini 5x100preps/kit

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FAQs

How to use GRS Plasmid Purification Kit – Mini:
Quality Control
The quality of the GRS Plasmid Purification Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4,5-ml overnight culture of Escherichia coli DH5α (OD600nm >2), harbouring pBluescript. Yields are typically over 20 μg of plasmid DNA with an A260/A280 ratio between 1.8 and 2.0. Subsequently, 1μg of the purified plasmid is digested with EcoRI, and fragments are analyzed by agarose gel electrophoresis.