HEPES Buffer Solution (1 M)

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is widely used as a buffering agent in cell culture media.

Description

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is widely used as a buffering agent in cell culture media. The zwitterion possesses a pKa(1) of 3 and a pKa(2) of 7.5 with useful pH ranges of 2.5-3.5 and 6.7-8.6. In comparison with bicarbonate buffer systems, HEPES is better in maintaining physiological pH despite changes in CO2 concentrations resulting from cellular activity. HEPES is suitable for many cell culture systems as it is membrane impermeable and has limited effect on biochemical reactions.

Quantity: 100ml
Appearance: Clear colourless solution.

Features

Storage: Shipped at room temperature
Store at +2ºC to +8ºC, protected from light.
Specifications: Concentration: 238.30 g/l (1.00M)
pH: 6.5–7.5
Osmolality: >1000 mOsm/kg
Endotoxin: <0.05 EU/ml (at 25mM concentration)
Sterility: sterile
Preparation of working solution: Add directly to cell culture media to a final concentration of 25-40 mM

Documents

Download Product Info Datasheet

Download MSDS GTC14 HEPES Buffer Solution (1M) v7E503

Order Information

GTC14.0100 HEPES Buffer Solution (1 M) 100mL