Kanamycin, from Streptomyces kanamyceticus, is a bactericidal agent that acts by binding to the 30S subunit inhibiting protein synthesis, and as such functions as a broad-spectrum antibiotic, effective against many Gram(+) and most Gram(-) bacteria. Kanamycin is frequently used for the selection of bacteria transformed with a vector harbouring a gene conferring kanamycin resistance, but it is also often used for the prevention of bacterial contamination of cell cultures.
Appearance: White, slightly off-white crystalline solid
Storage: 2ºC – 8ºC for up to 3 years.
Preparation: Prepare a stock solution of 10-50mg/ml in ultrapure water, and filter sterilize. Do not autoclave. Store stock solution at +4ºC for several weeks or at -20ºC for long term storage.
Usage: For incorporation in agar plates or broth, add kanamycin to a final concentration of 50 μg/ml (10-100μg/ml depending on, among other factors, type of vector (low-copy vs high-copy number plasmid)) by adding to autoclaved media agar, just prior to pouring the plates, or to broth after cooling down to room temperature.
Formula: C18H36N4O11 (+H2SO4)
MW: 528,6 g/mol
Potency: >750μg/g (on dry basis)
Water content: <4% (Karl Fischer)
Solubility (H2O): 50mg/ml