GRiSP

RapidClean

The fast and easy way to remove protein from RNA and DNA samples

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Description

The fast and easy way to remove protein from RNA and DNA samples

In less than 5 minutes, completely remove protein from aqueous solutions of single- and double-stranded nucleic acids.

Additional information

Quantidade

10 rxns, 25 rxns

Features

  • Complete removal of proteins from aqueous solutions of nucleic acids
  • Protocol less than 5 minutes, start to finish
  • Non-toxic alternative to phenol-chloroform extraction
  • Nucleic acid recovery rates >95%
  • Recovery of nucleic acids from 5 nucleotides to greater than 40 kb
  • Suitable for DNA, RNA, cDNA, microRNA, oligonucleotides

RapidClean is a novel affinity resin designed to remove all protein from aqueous solutions of nucleic acids, providing an alternative to hazardous and lengthy phenol-chloroform procedures. The RapidClean resin binds and removes protein in a 5-minute protocol that combines convenience, speed, and nucleic acid recovery rates in excess of 95%.

Figure 1
Figure 1. RapidClean completely removes T4 DNA ligase. No detectable ligase activity remains in a reaction mix after exraction with RapidClean (lane 2). EcoRI digested λ-DNA (lane C, Control) was incubated for 16 hours at room temperature with T4 DNA ligase (lane 1) or with the same mixture extracted with RapidClean (lane 2).

Figure 2
Figure 2. Extraction with RapidClean completely removes Exonuclease III. ?X174/HaeIII DNA (Lane M) was digested by a 40 min incubation at 37 °C with 10 units of Exonuclease III (lane 1), but no digestion occured when the enzyme mix was extracted twice with RapidClean before addition of the ?X174/HaeIII DNA target (lane 2).

Rapid clean resin arrives as a ready-to-use 10x slurry
Rapid clean kit Includes:
– RapidClean resin
– Spin filters

Documents

RapidClean Flyer

RapidClean Application Note

RapidClean ShortProtocol

RapidClean User Manual

Video

Order Information

Reference Description Quantity
K-01001-010 Rapid Clean Protein Removal Kit 1L 10X
K-01001-025 Rapid Clean Protein Removal kIT 1L 10X
R-14011-250 Rapid Clean Resin 250ml
R-14011-B10 Rapid Clean Resin 1ml

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FAQs

How to use RapidClean:
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