GRiSP

TAE Buffer (10X)

Tris-Acetate-EDTA, 10x concentrated buffer for electrophoresis

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Description

TAE Buffer (10X) is an aqueous solutions of 400mM Tris, 200mM acetic acid, and 10mM EDTA, prepared with ultrapure water, and 0.2 μm filtered. TAE is the most common buffer used for agarose gel electrophoresis in the analysis of DNA samples and is recommended for high resolution of large DNA fragments (>3kb) or supercoiled DNA, however, it can also be used for non?denaturating RNA agarose electrophoresis.

Usage
The working concentration is either 1X or 0,5X. Prepare 1L TAE Buffer (1X) by mixing 100ml of the 10x concentrated buffer with 900ml of ddH2O. A 1X TAE buffer consists of 40mM Tris?acetate, 1mM EDTA at pH 8.3±0.1. TAE should be used both for the preparation of the agarose gel as well as the running buffer. Note that compared with other electrophoresis buffers, such as TBE or SGTB, TAE has
a low ionic strength and low buffering capacity and may become exhausted during extended electrophoresis (>4h). The running buffer should therefore be replaced frequently.

Storage
TAE Buffer (10X) should be stored at room temperature and is stable for up to one and a half years.

Additional information

Quantidade

1L 10X, 5L 10X

Documents

Download Product Info Datasheet

Download MSDS GB11 TAE (10X) v7E502

Order Information

Reference Description Quantity
GB11.0110 TAE Buffer 1L 10X
GB11.0510 TAE Buffer 5L 10X

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FAQs

How to use TAE Buffer (10X):
Prepare 1x SGTB DNA electrophoresis buffer by diluting the concentrate in pure water | Prepare the agarose gel with 1x SGTB, as usual | Fill the electrophoresis chambers with 1x SGTB and put the gel casting tray into place (the first time, chambers need to be washed with 1x SGTB in order to remove residual previous buffer) | For optimal resolution, cover the gel with 3-5 mm of SGTB | Apply samples and run the gel at constant voltage, using a higher voltage than usual (GRiSP suggests doubling the voltage you normally use and optimize gradually) | The optimal voltage depends, among other factors, on the type and percentage of agarose, as well as on the nature of the sample. Therefore, occasionally the optimal voltage might be less than 2x the normal voltage | The run time will be reduced considerably!