Tetracycline is a bacteriostatic agent that acts by preventing aminoacyl tRNA from binding to the ribosome A site, thus inhibiting protein synthesis. It also binds to the bacterial 50S ribosomal subunit, causing leaking of intracellular components via the membrane. Tetracycline is broad-spectrum antibiotic, effective against Gram(+) and Gram(-) bacteria. Tetracycline is frequently used for the selection of bacteria transformed with a vector harbouring a gene conferring tetracycline resistance (Tn10), but it is also often used for the prevention of bacterial contamination of cell cultures.
Appearance: yellow crystalline solid
Storage: 2ºC–8ºC or -20ºC for at least to 3 years. Tetracycline is light sensitive and product as well as medium containing tetracycline should be stored in the dark.
Preparation: Prepare a stock solution of 10-12mg/ml in 70% ethanol, and filter sterilize. Do not autoclave. Store stock solution at -20ºC (stable at 37ºC for 3-4 days).
Usage: For incorporation in agar plates or broth, add tetracycline to a final concentration of 10-12μg/ml by adding to autoclaved media agar, just prior to pouring the plates, or to broth after cooling down to room temperature.
Formula: C22H24N2O8 (+HCL)
MW: 480,9 g/mol
Potency: >950μg/g (on dry basis)
Solubility: H2O, MeOH, EtOH
Specific optical rotation: ?240? to ?255?