TGS (Tris-Glycine-SDS) is the most common buffer used for sodium dodecyl sulfate – polyacrylamide gel electrophoresis of proteins (SDS-PAGE). TGS Buffer (10X) is an aqueous solutions of 0,25M Tris, 1,92M glycine, and 1% SDS, prepared with ultrapure water, and 0.2 μm filtered.
Usage
The working concentration is 1X TGS. Prepare 1L TGS Buffer (1X) by mixing 100ml of the 10x concentrated buffer with 900ml of ddH2O. A 1X TGS buffer consists of 25 mM Tris, 192 mM glycine, 0,1% SDS at pH 8.3. In SDS?PAGE, TGS is used as both anode and cathode buffer. Recommended running conditions for a mini vertical electrophoresis unit are 50V and 150V for stacking and running gel, respectively.
Storage
TGS Buffer (10X) should be stored at room temperature and is stable for up to one year.
References
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227: 680-685
| GB15.0110 | TGS buffer | 1L 10X |
| GB15.0510 | TGS buffer | 5L 10X |
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