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TGS Buffer (10X)

Tris-Glycine-SDS, 10x concentrated buffer

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Description

TGS (Tris-Glycine-SDS) is the most common buffer used for sodium dodecyl sulfate – polyacrylamide gel electrophoresis of proteins (SDS?PAGE). TGS Buffer (10X) is an aqueous solutions of 0,25M Tris, 1,92M glycine, and 1% SDS, prepared with ultrapure water, and 0.2 μm filtered.

Additional information

Quantidade

1L, 5L

Features

Usage
The working concentration is 1X TGS. Prepare 1L TGS Buffer (1X) by mixing 100ml of the 10x concentrated buffer with 900ml of ddH2O. A 1X TGS buffer consists of 25 mM Tris, 192 mM glycine, 0,1% SDS at pH 8.3. In SDS?PAGE, TGS is used as both anode and cathode buffer. Recommended running conditions for a mini vertical electrophoresis unit are 50V and 150V for stacking and running gel, respectively.

Storage
TGS Buffer (10X) should be stored at room temperature and is stable for up to one year.

Documents

Download Product Info Datasheet

Download MSDS GB15 TGS (10X) v7E502

Publications

References

Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227: 680-685

Order Information

Reference Description Quantity
GB15.0110 TGS buffer (10X) 1L
GB15.0510 TGS buffer (10X) 5l

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FAQs

How to use TGS Buffer (10X):
References
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680?685