GRiSP

Xpert AmpliFi

Xpert AmpliFi DNA Polymerase is an all-round and robust high-fidelity enzyme with enhanced DNA binding, resulting in improved processivity, shorter extension times, higher yield and capacity to amplify more difficult and longer targets. Combined with its extremely low error-rate, this makes Xpert AmpliFi ideal for applications such as high-fidelity PCR, site-directed mutagenesis, cloning, long-range PCR and DNA sequencing, when robustness and proof-reading are extremely important.

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Description

Xpert AmpliFi DNA Polymerase is an all-round and robust high-fidelity enzyme with enhanced DNA binding, resulting in improved processivity, shorter extension times, higher yield and capacity to amplify more difficult and longer targets. Combined with its extremely low error-rate, this makes Xpert AmpliFi ideal for applications such as high-fidelity PCR, site-directed mutagenesis, cloning, long-range PCR and DNA sequencing, when robustness and proof-reading are extremely important.

Xpert AmpliFi DNA polymerase has been derived from Pfu DNA polymerase for its proof-reading activity. Xpert AmpliFi has a very high accuracy resulting in fidelity that is about 100x higher than regular Taq DNA polymerase. DNA binding has been improved by protein engineering resulting in shorter extension times (2-6kb/min), making this also an ideal choice for consistent results in fast PCR amplifications.

Additional information

Quantidade

1.25mL, 100U, 5x 1.25mL

Features

Properties:
Amplicon size:  up to 20kb
Extension Rate: 2-6kb/min
Hotstart:           No
A-overhang:      No
Error-rate:  100x lower than Taq

Applications:
– High-fidelity PCR
– Complex targets (including GC-rich and AT-rich templates)
– Longe-Range PCR
– site-directed mutagenesis
– DNA Sequencing
– Cloning

Components:
– Kit version with dNTPs in separated buffer or
– Mastermix

Storage:
-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.

Documents

Product Info Xpert AmpliFi (Kit)

Product Info Xpert AmpliFi Mastermix

Order Information

Reference Description Quantity
GE17.0100 Xpert AmpliFi 100U
GE27.0100 Xpert AmpliFi Mastermix 1.25ml
GE27.5100 Xpert AmpliFi Mastermix 5x 1.25ml

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Product: Xpert AmpliFi

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FAQs

How to use Xpert AmpliFi:
Functionally tested in PCR. Bacterial DNA free (tested by PCR). Absence of endonucleases, exonucleases, and ribonucleases was confirmed by appropriate assays.