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Xpert Fast Probe

For high performance, fast cycling, qPCR using Probe Xpert Fast Probe (uni) 2X mastermix contains all components, except primers and template, for the amplification and detection of DNA in qPCR based on a wide range of probe-based technologies.

 

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Description

Main application: extremely high sensitivity and specificity qPCR with Probe

Xpert Fast Probe (uni) 2X mastermix contains all components, except primers and template, for the amplification and detection of DNA in qPCR based on a wide range of probe-based technologies, including Taqman®, Molecular Beacons® and Scorpion probes®. It consists of the combination of a highly efficient enzyme with a novel low inhibitory technology.

Xpert Fast Probe (uni) can be used to quantify virtually any DNA target, including extremely low-copy number targets, with minimal effort and optimization.

Xpert Fast Probe (uni) 2X mastermix is supplied with a separate vial of ROX reference dye, so it can be used with most real-time PCR instruments.

Additional information

Quantidade

1 mL (100 rxns), 25x 1mL (250rxn), 5x 1mL (500 rxns)

Features

Properties:
– Early Ct values – Rapid extenstion rate
– High Efficiency in multiplex reactions
– High Efficiency in GC or AT-rich templates
– Extreme sensitivity – increased limit of detection
– Allows for standard and fast cycling
– Compatible with most qPCR machines (see FAQs)

Applications:
– Absolute quantification
– Gene expression analysis
– Diagnostic real-time PCR
– Low-copy number target genes
– Multiplex or singleplex

Components:
Xpert Fast Probe is supplied in multiples of 1mL, for your convenience!

Xpert Fast Probe is supplied as a convenient 2x mastermix containing all required components except specific primers. 1mL is enough for 100 reactions of 20μl. The mastermix is supplied with a separate vial of 100X (50µM) ROX solution. Depending on the PCR equipment (see FAQs), prior to use, one should add 2µl of ROX (LowROX (final concentation of 50nM), 20µl (HighROX (final concentation of 500nM)), or nothing (No ROX). Once ROX has been added, the mastermix might be used immediately or stored at -20ºC.

Storage:
-20ºC for at least 1 year

Documents

Xpert qPCR selection table

Product Info Xpert Fast Probe

 

Order Information

Reference Description Quantity
GE30.0100 Xpert Fast Probe (uni) 1mL
GE30.5100 Xpert Fast Probe (uni) 5x 1mL
GE30.2501 Xpert Fast Probe (uni) 25x 1mL

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Product: Xpert Fast Probe

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FAQs

How to use Xpert Fast Probe:
Q: How much ROX should I add to Xpert Fast Probe (uni) ?
A: This depends on whether your instrument is “No ROX”, “LowROX”, or “HighROX” (see table below), In case of “No ROX”, one should use the mastermix as is, thus without adding additional ROX. In case of “LowROX”, one should add 2µl of 100X ROX solution (supplied) to 1ml of mastermix, and in case of “HighROX”, one should add 20µl of 100X ROX solution to 1ml of mastermix. Once ROX has been added, the mastermix can be used directly or stored at -20ºC for up to 1 year. If your instrument is not listed, please feel free to contact us.

Xpert Fast Probe (uni) Selection Table:

Manufacturer Model No ROX LowROX HighROX
Agilent/Stratagene MX3000P® X
MX3005P® X
MX4000P® X
BioRad CFX96™ X
CFX384™ X
Cromo4™ X
MiniOpticon™ X
Opticon™ X
Opticon™2 X
Illumina Eco™ X
Life Technologies ABI7000 X
ABI7300 X
ABI7500 X
ABI 7500 Fast X
ABI 7700 X
ABI 7900 X
ABI 7900HT X
ABI 7900HT Fast X
QuantStudio™12k Flex X
StepOne™ X
StepOne™Plus X
ViiA7™ X
Qiagen Rotor-Gene™ 3000 X
Rotor-Gene™ 6000 X
Rotor-Gene™ Q X
Roche LightCycler™ 96 X
LightCycler™ 480 X
LightCycler™ nano X


Q: Can I use Xpert Fast Probe (uni) with instruments that use fluorescein as a reference dye ?
A: No.