GRiSP

Xpert Fast SYBR

For high performance, fast cycling, qPCR with SYBR Xpert Fast SYBR consists of the combination of a highly efficient enzyme with a novel low inhibitory technology, allowing for extremely high sensitivity and specificity.

 

Clear

Description

Main application: extremely high sensitivity and specificity qPCR with SYBR

Xpert Fast SYBR (uni) 2X mastermix contains all components, except primers and template, for the amplification and detection of DNA in qPCR. It consists of the combination of a highly efficient enzyme with a novel low inhibitory technology. The intercalating dye used in this mastermix causes little to no inhibition of the PCR reaction thus allowing for extremely high sensitivity and specificity, as well as preventing the formation of unwanted primer-dimers and non-specific products.

Xpert Fast SYBR (uni) can be used to quantify virtually any DNA target, including extremely low-copy number targets, with minimal effort and optimization.

Xpert Fast SYBR (uni) 2X mastermix is supplied with a separate vial of ROX reference dye, so it can be used with most real-time PCR instruments.

Additional information

Quantidade

(fluorescein) 1ml (100 rxns), (fluorescein) 5x 1ml (500 rxns), 1 mL (100 rxns), 25x 1mL (2500 rxns), 5x 1mL (500 rxns)

Features

Properties:
– Excellent signal with low PCR inhibition
– Early Ct values – Rapid extenstion rate
– Extreme sensitivity – increased limit of detection
– Allows for standard and fast cycling
– Compatible with all qPCR machines (see FAQs for compatibility)

Applications:
– Absolute quantification
– Gene expression analysis
– High throughput qPCR
– Low-copy number target genes

Performance:
 

Amplification and melt trace of ACTG1 (Actin Gamma-1). Comparison between Xpert Fast SYBR (orange), and Market Leader (black).

Components:
All Xpert Fast SYBR versions and sizes are supplied in multiples of 1mL, for your convenience!

– Xpert Fast SYBR (uni)
Xpert Fast SYBR (uni) is supplied as a convenient 2x mastermix containing all required components except specific primers. 1mL is enough for 100 reactions of 20μl.
Supplied also with a separate vial of 100X (50µM) ROX solution. Depending on the PCR equipment (see FAQs), prior to use, one should add 2µl of ROX (LowROX (final concentation of 50nM), 20µl (HighROX (final concentation of 500nM)), or nothing (No ROX). Once ROX has been added, the mastermix might be used immediately or stored at -20ºC.

– Xpert Fast SYBR (fluorescein)
Xpert Fast SYBR (fluorescein) is supplied as a convenient 2x mastermix containing all required components, including 20nM fluorescein, except specific primers. 1mL is enough for 100 reactions of 20μl.
This mastermix is for instruments equipped with a well-factor correction feature (see FAQs).

Storage:
-20ºC for at least 1 year.

Documents

Datasheet – Xpert Fast SYBR (uni)

Datasheet – Xpert Fast SYBR (fluorescein)

Xpert flyer

Xpert qPCR selection table

Order Information

Reference Description Quantity
GE20.0100 Xpert Fast SYBR (Uni) 1ml (100rxns))
GE20.5100 Xpert Fast SYBR (Uni) 5x 1ml (500rxns)
GE20.2501 Xpert Fast SYBR(Uni) 25x1ml (2500rxns)
GE21.0100 Xpert Fsat SYBR (Flurescein) 1ml(100rxns)
GE21.5100 Xpert Fsat SYBR (Flurescein) 5x 1ml (500rxns)

Ask a Question

Note that by submitting this contact form, you are providing GRISP with some personal information. We encourage you to take the time to review our revised Privacy Policy.

Product: Xpert Fast SYBR

Request Price for Xpert Fast SYBR

FAQs

How to use Xpert Fast SYBR:
Q: How much ROX should I add to Xpert Fast SYBR (uni) ?
A: This depends on whether your instrument is “No ROX”, “LowROX”, or “HighROX” (see table below), In case of “No ROX”, one should use the mastermix as is, thus without adding additional ROX. In case of “LowROX”, one should add 2µl of 100X ROX solution (supplied) to 1ml of mastermix, and in case of “HighROX”, one should add 20µl of 100X ROX solution to 1ml of mastermix. Once ROX has been added, the mastermix can be used directly or stored at -20ºC for up to 1 year. If your instrument is not listed, please feel free to contact us.

Xpert Fast SYBR Selection Table:

Manufacturer Model No ROX LowROX HighROX
Agilent/Stratagene MX3000P® X
MX3005P® X
MX4000P® X
BioRad CFX96™ X
CFX384™ X
Cromo4™ X
MiniOpticon™ X
Opticon™ X
Opticon™2 X
Illumina Eco™ X
Life Technologies ABI7000 X
ABI7300 X
ABI7500 X
ABI 7500 Fast X
ABI 7700 X
ABI 7900 X
ABI 7900HT X
ABI 7900HT Fast X
QuantStudio™12k Flex X
StepOne™ X
StepOne™Plus X
ViiA7™ X
Qiagen Rotor-Gene™ 3000 X
Rotor-Gene™ 6000 X
Rotor-Gene™ Q X
Roche LightCycler™ 96 X
LightCycler™ 480 X
LightCycler™ nano X
Q: When should I use Xpert Fast SYBR (fluorescein) ?
A: Xpert Fast SYBR (fluorescein) has been developed for instruments equipped with a well-factor correction feature, These include BioRad´s iCYCLER®, IQ™5, and MyiQ™. If your instrument is not listed, please feel free to contact us.