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Xpert Hotstart DNA polymerase

For amplification of complex targets; for multiplex Xpert Hotstart DNA polymerase is a chemically modified hotstart Taq DNA polymerase, with excellent amplification efficiency.

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Description

Main application: amplification of complex targets; multiplex

Xpert Hotstart DNA polymerase is a chemically modified hotstart Taq DNA polymerase, with excellent amplification efficiency, enabling higher specificity, increased sensitivity, and greater yield, as compared to standard Taq DNA polymerases, making this the ideal choice for consistent results in complex PCR amplifications and multiplex PCR.

Additional information

Quantidade

(+dNTPs) 500 U, 1.25mL, 500 U, 5x 1.25mL, with dye (100rxn) 1.25mL, with dye (500rxn) 5x 1.25m

Features

Performance highlights:
– minimization of non-specific amplification
– high sensitivity, even for complex targets

Properties:
Amplicon size:  up to 5kb
Extension Rate: 2kb/min
Hotstart:           Yes
A-overhang:      Yes

Applications:
– Complex targets (including GC-rich and AT-rich templates)
– Multiplex PCR

Components:
– Kit version with dNTPs included (separate vial) or without dNTPs
– Mastermix available with (Blue) dye, and without dye

Storage:
-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.

Documents

Product Info – Xpert Hotstart Mastermix Download

Product Info – Xpert Hotstart Mastermix with dye

Product Info – Xpert Hotstart DNA Polymerase (with dNTPs)

Order Information

Reference Description Quantity
GE08.0500 Xpert Hotstart DNA polymerase (+dNTPs) 500U
GE18.0100 Xpert Hotstart 2X Mastermix (100rxn) 1.25ml
GE18.5100 Xpert Hotstart 2X Mastermix (500rxn) 5×1.25ml
GE28.0100 Xpert Hotstart 2X Mastermix with dye (100rxn) 1.25ml
GE28.5100 Xpert Hotstart 2X Mastermix with dye (500rxn) 5×1.25ml
GE48.0500 Xpert Hotstart DNA polymerase 500U

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Product: Xpert Hotstart DNA polymerase

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FAQs

How to use Xpert Hotstart DNA polymerase:
Functionally tested in PCR. Absence of endonucleases, exonucleases, and ribonucleases was confirmed by appropriate assays.