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Xpert One-Step Fast SYBR

Using gene-specific primers (GSP), Xpert One-Step Fast SYBR allows for first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably. Xpert One-Step Fast SYBR consists of a RTase mix, a Fast qPCR Mastermix (SYBR), and separated vial(s) of ROX

 

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Description

Using gene-specific primers (GSP), Xpert One-Step Fast SYBR allows for first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably. Xpert One-Step Fast SYBR consists of a RTase mix, a Fast qPCR Mastermix (SYBR), and separated vial(s) of ROX

The RTase mix comprises a genetically modified thermostable MMLV reverse transcriptase with improved synthesis efficiency, lacking any RNase H activity, and an advanced RNase inhibitor to impede RNA degradation.

The Fast qPCR Mastermix (SYBR) consists of the combination of a highly efficient enzyme with a novel low inhibitory technology. The intercalating dye used in this mastermix causes little to no inhibition of the PCR reaction thus allowing for extremely high sensitivity and specificity, as well as preventing the formation of unwanted primer-dimers and non-specific products. Xpert One-Step Fast SYBR can be used to quantify virtually any RNA target, whether using mRNA, viral RNA or total RNA as template, including extremely low-copy number targets, with minimal effort and optimization.

Additional information

Quantidade

100 rxn, 5x 100 rxn

Features

Applications: 
One-Step RT-qPCR,
Absolute quantification,
Gene expression analysis,
Low-copy number target gene detection.

Properties:
Excellent signal with low PCR inhibition
Early Ct values – Rapid extension rate
Allows for standard and fast cycling

Samples:
t
otal RNA: 10pg – 100ng, mRNA: >0.01pg

Storage:
-20ºC for at least 1 year. Repeated thaw/freeze cycles (up to 25) have no negative impact on performance.

Documents

Download Product Info Datasheet 

Download MSDS GE40 Xpert One-Step Fast SYBR v7E503

Order Information

Reference Description Quantity
GE40.0100 Xpert One-Step Fast SYBR 100 rxn
GE40.5100 Xpert One-Step Fast SYBR 5x 100 rxn

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