Using gene-specific primers (GSP), Xpert One-Step Fast SYBR allows for first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably. Xpert One-Step Fast SYBR consists of a RTase mix, a Fast qPCR Mastermix (SYBR), and separated vial(s) of ROX
The RTase mix comprises a genetically modified thermostable MMLV reverse transcriptase with improved synthesis efficiency, lacking any RNase H activity, and an advanced RNase inhibitor to impede RNA degradation.
The Fast qPCR Mastermix (SYBR) consists of the combination of a highly efficient enzyme with a novel low inhibitory technology. The intercalating dye used in this mastermix causes little to no inhibition of the PCR reaction thus allowing for extremely high sensitivity and specificity, as well as preventing the formation of unwanted primer-dimers and non-specific products. Xpert One-Step Fast SYBR can be used to quantify virtually any RNA target, whether using mRNA, viral RNA or total RNA as template, including extremely low-copy number targets, with minimal effort and optimization.