Description
Using gene-specific primers (GSP), the Xpert One-Step RT-PCR Kit allows for first-strand cDNA synthesis and subsequent PCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably.
The kit consists of a RTase mix and a Fast PCR Mastermix. The RTase mix comprises a genetically modified thermostable MMLV reverse transcriptase with improved synthesis efficiency, lacking any RNase H activity, and an advanced RNase inhibitor to impede RNA degradation. The Fast PCR Mastermix contains all other required components, including a fast hot-start DNA polymerase for improved speed, sensitivity and specificity, MgCl2, dNTPs, enhancers and stabilizers.
The Xpert One-Step RT PCR Kit can be used with used with any RNA template, including mRNA, viral RNA and total RNA, as the RTase is not inhibited by rRNA or tRNA. The Kit provides a robust RT-PCR performance that requires minimal or no optimization. PCR products contain 3´A-overhang and are thus suitable for TA cloning.
