GRiSP

Xpert qDetect COVID-19

GRiSP´s Xpert qDetect COVID-19 is a qRT-PCR detection kit for SARS-CoV-2, based on real-time PCR assays for N1, N2 and RP. The US Centers for Disease Control and Prevention (CDC) has developed and published a protocol for the detection of SARS-CoV-2 (2019-nCoV). This kit combines GRiSP´s highly efficient, fast and sensitive Xpert One-Step Fast Probe for qRT-PCR with the published primers and probes[1] from these CDC assays for the identification of this new coronavirus.

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Description

GRiSP’s Xpert qDetect COVID-19 is a qRT-PCR kit for the detection of SARS-CoV-2, based on real-time PCR assays for N1N2 and RP. It is for the use of COVID19 screening tests or the evaluation of anti-viral drug efficacy. Selected targets, corresponding primer sequences and probes are according to the protocol[1] published by the US CDC. Check your National government´s guidelines and recommendations to see which specific viral targets should be detected in your country, as they may be different. This product is for research only (RUO) and is to be used by trained laboratory professionals only.

GRiSP’s Xpert qDetect COVID-19 is intended for the in vitro detection and quantification of SARS-CoV-2 RNA, previously isolated from human nasopharyngeal/tracheal/oropharyngeal apirates, swabs or washes, or sputum specimens (among others), in a one-step qRT-PCR reaction targeting 2 viral targets (N1 and N2) using viral target specific primers and Taqman probe technology

Figure 1. Schematic representation of the SARS-CoV-2 genome. ORF=open reading frame, S=Spike Protein gene. E=envelope protein gene, M=Membrane protein gene, and N=nucleocapsid protein. The gene encoding the RNA-dependent RNA polymerase (RdRp) is located on ORF1ab. The arrows indicate the viral targets of the Xpert Detect COVID-19 kit.

The human RNase P gene (RP) is used both as internal and extraction control to evaluate viral RNA extraction efficiency as well as to control qRT-PCR performance. Primer and Probe sequences can be found in the appendix. Primers and probes have been optimized to run under the same cycling conditions, thus analysis for N1, N2 and RP can be done in a single run (see also section 9: recommended pipetting scheme). All probes are FAM-labeled; hence this kit is to be used with real-time PCR instruments equipped with a FAM-channel.

Additional information

Quantidade

~100 samples, ~500 samples

Documents

Product Info GDK99.3100

Product Info GDK99.3500

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Order Information

Reference Description Quantity
GDK99.3100 Xpert qDetect COVID-19 3x 100 qRT-PCR reactions ~100 samples
GDK99.3500 Xpert qDetect COVID-19 3x 100 qRT-PCR reactions ~500 samples

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