GRiSP

Xpert TA Easy Cloning Kit

Xpert TA Easy cloning kit for direct ligation of PCR fragments containing 3´-A overhangs (generated using non-proofreading DNA polymerases such as Taq or blends thereof) into a ready-to-use stable linearized vector.

 

 

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Description

The Xpert TA Easy cloning kit is designed for the direct ligation of PCR fragments containing 3´-A overhangs (generated using non-proofreading DNA polymerases such as Taq or blends thereof) into a ready-to-use stable linearized vector. The vector is prepared by digestion of pGRS TA Easy cloning vector with EcoRV and the subsequent addition of a single thymidine at each of the 3´-ends. This system offers greater efficiency than most TA vectors available (with over 700 positive colonies under optimal conditions) and with a low background (less than 4%) due to reduced self-ligation.

Additional information

Quantidade

10 rxn, 20 rxn

Features

The pGRS TA Easy vector (3015bp) contains the β-lactamase gene resulting in ampicillin resistance for selection purposes. The PCR amplicon is inserted between pUC/M13 primers, which facilitates DNA sequencing. Flanking Lac, and dual opposed SP6/T7 promoters ensure the possibility for correct in vitro transcription of the insert, independently of its orientation. Flanking EcoRI and NotI recognition sites enable single enzyme digestion for subcloning.

Documents

Product Info Xpert TA Easy Cloning kit

pGRS TA+ Complete Sequence

Photos

Order Information

Reference Description Quantity
GC05.0010 Xpert TA Easy Cloning Kit 10rxn
GC05.0020 Xpert TA Easy Cloning Kit 20rxn

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