Description
The GRS Genomic DNA Kit – Card provides an easy and efficient method for the purification of high quality genomic DNA from dried blot spots (on Whatman® FTA® Cards).
Eluted purified DNA (approximately 20-30kb) is suitable for PCR, real-time PCR, and other enzymatic reactions.The GRS Genomic DNA Kit – Card uses proteinase K and chaotropic salts to lyse cells and to denature proteins. Carrier RNA is included to minimize DNA loss to plasticware. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column. Contaminants such as proteins, divalent cations, unincorporated nucleotides, and enzyme inhibitors are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified genomic DNA is subsequently eluted by a low salt Elution Buffer or TE or water.
The entire procedure can be completed within an hour without phenol/chloroform extraction or alcohol precipitation, with typical DNA yields of 300ng from a 6mm dried blot spot.