Acrylamide/Bisacrylamide Solutions (19:1)

Ready-to-Use Acrylamide-Bisacrylamide solutions are aqueous solutions of ultrapure, 3x re-crystallized acrylamide and molecular biology grade bisacrylamide, prepared with ultrapure water, 0.2 µm filtered.

 

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Description

Ready-to-Use Acrylamide-Bisacrylamide solutions are aqueous solutions of ultrapure, 3x re-crystallized  acrylamide and molecular biology grade bisacrylamide, prepared with ultrapure water, 0.2 µm filtered,  and suitable for electrophoresis of proteins and nucleic acids.
The concentration of this product (30% or 40%) is determined by the total (T) weight of both acrylamide and bisacrylamide (T= 30g or T=40g, per 100ml), in which the mix ratio is 19:1 resulting in a cross-linking (C) of 5%.

 

Features

Principle
The range of  protein sizes to be separated is determined by the pore size of a polyacrylamide gel. Control of the pore size is accomplished by regulating both T and C. The relationship between T and pore size is nearly linear; Gels with a higher percentage of T have smaller pores, and are used to separate smaller proteins. However, the relationship between C and pore size is more complex with a minimum occurring at about 5% bisacrylamide. A ratio between acrylamide and bisacrylamide of 19:1 (5% C) is suitable for the separation of small peptides, whereas a ratio of 29:1 [this product] is commonly used for the separation of “normal sized” proteins. High molecular weight proteins are best separated using a 37,5:1 mix ratio.

Download the protocol for more informations

Documents

Download Product Info Datasheet – Acrylamide Bisacrylamide 30% (19:1)

Download Product Info Datasheet – Acrylamide Bisacrylamide 40% (19:1)

MSDS Acrylamide-Bisacrylamide Solutions

Order Information

GB16.3019 Acrylamide/Bisacrylamide Solution 30% (19:1) 500mL
GB16.4019 Acrylamide/Bisacrylamide Solution 40% (19:1) 500mL