Acrylamide /Bisacrylamide Solutions (37.5:1)

Ready-to-Use Acrylamide-Bisacrylamide solutions are aqueous solutions of ultrapure, 3x re-crystallized acrylamide and molecular biology grade bisacrylamide, prepared with ultrapure water, 0.2 µm filtered, and suitable for electrophoresis of protein

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Description

Ready-to-Use Acrylamide-Bisacrylamide solutions are aqueous solutions of ultrapure, 3x re-crystallized  acrylamide and molecular biology grade bisacrylamide, prepared with ultrapure water, 0.2 µm filtered,  and suitable for electrophoresis of proteins and nucleic acids.
The concentration of this product (30% or 40%) is determined by the total (T) weight of both acrylamide and bisacrylamide (T= 30g or T=40g, per 100ml), in which the mix ratio is 37.5:1 resulting in a cross-linking (C) of 2.7%.

 

Features

Principle
The range of  protein sizes to be separated is determined by the pore size of a polyacrylamide gel. Control of the pore size is accomplished by regulating both T and C. The relationship between T and pore size is nearly linear; Gels with a higher percentage of T have smaller pores, and are used to separate smaller proteins. However, the relationship between C and pore size is more complex with a minimum occurring at about 5% bisacrylamide. A ratio between acrylamide and bisacrylamide of 19:1 (5% C) is suitable for the separation of small peptides, whereas a ratio of 29:1 [this product] is commonly used for the separation of “normal sized” proteins. High molecular weight proteins are best separated using a 37,5:1 mix ratio.

?Download the protocol for more informations

Documents

Product Info – Acrylamide Bisacrylamide Solution 4

Product Info – Acrylamide Bisacrylamide Solution 3

Protocols

1. After Western Blotting, image the blot for permanent record, and then rinse the blot with 20ml of water for 5 minutes at room temperature, using an orbital shaker.
2. Decant and incubate the membrane in a container containing 10-20ml of GRS Stripping Buffer (membrane must be completely covered) at room temperature for 5-10 minutes, using an orbital shaker
3. After stripping, wash the membrane extensively (min 3x) with wash buffer (e.g. TBS(T) or PBS(T)).
4. After washing, block the membrane as usual.
5. After blocking the membrane is ready for the next detection with other set of antibodies.

Note: Because Stripping may reduce signal intensity, it is recommended to probe first for the antigen with the lowest level of expression. If more stringent stripping is required, one could add reducing agent (DTT or β-mercaptoethanol) to the stripping buffer and/or heat the blot during the stripping procedure.

Order Information

GB16.3037 Acrylamide/Bisacrylamide Solution 30% (37,5:1) 500mL
GB16.4037 Acrylamide/Bisacrylamide Solution 40% (37,5:1) 500mL