The Exo/SAP Go – PCR purification kit is an enzymatic PCR Clean-Up kit, comprising Exonuclease I (Exo I) and recombinant Shrimp Alkaline Phosphatase (rSAP) in an optimal molar ratio. Unused primers are hydrolyzed by Exo I, whilst rSAP dephosphorylates excess dNTPs.
Exo/SAP Go is designed to degrade dNTPs and primers in a single reaction tube in only 5 minutes. Both enzymes are subsequently removed by heat-inactivation and DNA is ready for sequencing in 15 minutes.
No further treatment is required and recovery is 100%, even for very short PCR products.