GRiSP

Exo/SAP Go - PCR Purification kit

The Exo/SAP Go – PCR purification kit is an enzymatic PCR Clean-Up kit, comprising Exonuclease I (Exo I) and recombinant Shrimp Alkaline Phosphatase (rSAP) in an optimal molar ratio.
Clear

Description

 

The Exo/SAP Go  – PCR purification kit is an enzymatic PCR Clean-Up kit, comprising Exonuclease I (Exo I) and recombinant Shrimp Alkaline Phosphatase (rSAP) in an optimal molar ratio.  Unused primers are hydrolyzed by Exo I, whilst rSAP dephosphorylates excess dNTPs.

Exo/SAP Go is designed to degrade dNTPs and primers in a single reaction tube in only 5 minutes. Both enzymes are subsequently removed by heat-inactivation and DNA is ready for sequencing in 15 minutes.

No further treatment is required and recovery is 100%, even for very short PCR products.

 

 

Additional information

Quantidade

4x500rnxs, 500rnxs

Documents

Product-Info – ExoSAP Go

 

 

Photos

Order Information

Reference Description Quantity
GK18.0500 Exo/SAP Go – PCR purification kit 500rnxs
GK18.2000  Exo/SAP Go – PCR purification kit 4x500rnxs

Ask a Question

Note that by submitting this contact form, you are providing GRISP with some personal information. We encourage you to take the time to review our revised Privacy Policy.

Product: Exo/SAP Go - PCR Purification kit

Request Price for Exo/SAP Go - PCR Purification kit

FAQs

How to use Exo/SAP Go – PCR Purification kit:
PROTOCOL

1) Transfer 5µl of PCR product to a new tube
2) Add 1µl of rSAP and 1µl of Exo I
3) Incubate at 37ºC for 5 minutes
4) Heat-inactivate at 80ºC for 10 minutes

The purified PCR product can be used for DNA sequencing reaction immediately