The GRS Genomic DNA Kit – Bacteria – provides an efficient and fast method for the purification of high quality genomic (and viral) DNA from Gram-positive and Gram-negative bacteria, suitable for all common downstream applications such as PCR, enzymatic restriction digestion, cloning, Southern blot analysis, etc.
The GRS Genomic DNA Kit – Bacteria – can be used for both Gram (-) and Gram (+) bacterial cells. The provided Buffer G+, once supplemented with lysozyme, will lyse bacterial cell walls consisting of peptidoglycan. Chaotropic salt is then used for further cell lysis and protein degradation. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column (1). Contaminants such as proteins, divalent cations, secondary metabolites, and enzyme inhibitors are completely removed using Wash Buffer. The purified genomic DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed in less than 60 minutes without phenol/chloroform extraction or alcohol precipitation, with a typical DNA yield of 25-30 μg from 1, 5ml of Escherichia coli (~109 cells).