Description
The GRS Genomic DNA Kit – Blood & Cultured Cells – provides an efficient and fast method for the purification and or concentration of high quality total DNA (including genomic DNA, mitochondrial DNA and viral DNA) from a variety of samples, including whole and frozen blood, buffy coat and cultured animal cells. Eluted purified DNA (approximately 20-30kb) is suitable for PCR, and other enzymatic reactions.
Principle
The GRS Genomic DNA Kit – Blood & Cultured Cells – uses chaotropic salts to lyse cells and to denature proteins. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column (1). Contaminants such as proteins, divalent cations, unincorporated nucleotides, and enzyme inhibitors are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified genomic DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed within an hour without phenol/chloroform extraction or alcohol precipitation, with typical DNA yields of 6μg from 200μl of whole human blood or up to 50μg from 200μl of buffy coat.