Description
The GRS Genomic DNA Kit – Food – provides an efficient and fast method for the purification of high-quality DNA from a broad variety of raw and processed food samples, suitable for all common downstream applications such as PCR, enzymatic restriction digestion, cloning, Southern blot analysis, etc.
The GRS Genomic DNA Kit – Food – can be used for both raw and processed food samples. When combined with Proteinase K, the specific lysis buffer (containing CTAB), results in the digestion of food tissues and proteins. Common PCR inhibitors (polysaccharides and plant metabolites) are removed by centrifugation whereas residual inhibitors are separated by chloroform (not included). The cleared supernatant is subsequently mixed with a binding buffer that is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column. Remaining contaminants are completely removed using Wash Buffer. The purified genomic DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed in less than 60 minutes without phenol/chloroform extraction or alcohol precipitation.