GRiSP

GRS Genomic DNA Kit - Plant

The GRS Genomic DNA Kit – Plant – provides an efficient and fast method for the purification and or concentration of high quality total DNA (including genomic DNA, mitochondrial DNA, and chloroplast DNA) from plant tissue and cells.

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Description

The GRS Genomic DNA Kit – Plant – provides an efficient and fast method for the purification and or concentration of high quality total DNA (including genomic DNA, mitochondrial DNA, and chloroplast DNA) from plant tissue and cells. Eluted purified DNA is suitable and ready-to-use for PCR, real-time PCR, Southern Blotting and RFLP.

Principle
Samples are ground in liquid nitrogen and further disrupted by incubation in lysis buffer. The lysate is treated with RNAse A to degrade RNA and subsequently filtered to remove cell debris and other precipitates. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column (1). Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified DNA is subsequently eluted with a low salt Elution Buffer (or TE). The entire procedure can be completed within 60 minutes without phenol extraction or ethanol precipitation with typical DNA yields of 5-20μg (up to 50μg).

Additional information

Quantidade

100 preps/kit

Features

Sample : up to 100mg of fresh plant tissue, up to 25 mg of dry plant tissue
Expected Yield : 3-5μg total DNA from 100mg Arabidopsis thaliana young leaf samples 20-25μg total DNA from 100mg Nicotiana tabacum young leaf samples
Format : spin column
Operation Time : within 60 minutes
Elution Volume : 50-200μl

Documents

Download Product Info Datasheet

Download MSDS GK04 GRS Genomic DNA Kit – Plant v7E0502

Order Information

Reference Description Quantity
GK04.0100 GRS Genomic DNA Kit – Plant 100 preps/kit

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Product: GRS Genomic DNA Kit - Plant

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FAQs

How to use GRS Genomic DNA Kit – Plant:
Quality Control
The quality of the GRS Genomic DNA Kit – Plant – is tested on a lot-to-lot basis by isolating total DNA from 50mg young leaf sample. Quantity and Quality are ascertained by spectroscopy and gel electrophoresis.