Description
The GRS Genomic DNA Kit – Soil – provides an efficient and fast method for the extraction and purification of high-quality genomic DNA from microorganisms (archaea, bacteria, fungi, and algae) found in soil samples.
Samples are disrupted and homogenized by vortexing in a lysis buffer mixed with zirconia/silica beads. Humic substances (HS), including insoluble particles, proteins and PCR inhibitors such as humic acid (HA), are precipitated using a unique buffer. Residual HA is subsequently removed using specific HA removal columns. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified DNA is subsequently eluted with a low salt Elution Buffer (or TE). The entire procedure can be completed within 40 minutes without phenol extraction or ethanol precipitation. Eluted purified DNA is suitable and ready-to-use for PCR, real-time PCR, Southern Blotting, RFLP and sequencing.