GRiSP

GRS PCR & Gel Band Purification Kit

The GRS PCR & Gel Band Purification Kit provides an efficient and fast method for the purification and or concentration of high quality DNA fragments (100bp to 10kb) from PCR reactions, enzymatic restriction digestion or from agarose gels.

Clear

Description

The GRS PCR & Gel Band Purification Kit provides an efficient and fast method for the purification and or concentration of high quality DNA fragments (70bp to 15kb) from PCR reactions, enzymatic restriction digestion or from agarose gels. Eluted DNA is suitable for all common downstream applications including PCR, enzymatic restriction digestion, cloning, library construction, Southern blot analysis, and DNA sequencing.

Principle
The GRS PCR & Gel Band Purification Kit uses chaotropic salts to dissolve agarose gel and to denature enzymes such as restriction enzymes or polymerases. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column (1). Contaminants such as proteins, divalent cations, unincorporated nucleotides, and enzyme inhibitors are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified genomic DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed in approximately 20 minutes without phenol/chloroform extraction or alcohol precipitation, with a typical DNA recovery of 80% to 90% for agarose gel extraction and up to 95% for PCR or Restriction Enzyme reactions.

 

Additional information

Quantidade

100 preps/kit, 3×100 preps/kit, 5×100 preps/kit

Features

Sample : up to 100μl of PCR products or DNA fragments from enzymatic reactions | up to 300mg of agarose gel
Expected Yield : typical recoveries up to 95% (PCR Clean-up) or up to 90% (Gel Extraction)
Format : spin column
Operation Time : 10 minutes (PCR clean-up), 20 minutes (Gel Extraction)
Elution Volume : 20-50 μl

Documents

Product Info –  GRS PCR &Gel Bnad Purification kit

MSDS-GRS PCR& Gel Band Purification Kit

Order Information

Reference Description Quantity
GK01.0100 GRS PCR &Gel Bnad Purification kit 100preps/kit
GK01.3100 GRS PCR &Gel Bnad Purification kit 3x 100preps/kit
GK01.5100 GRS PCR &Gel Bnad Purification kit 5x100preps/kit

Ask a Question

Note that by submitting this contact form, you are providing GRISP with some personal information. We encourage you to take the time to review our revised Privacy Policy.

Product: GRS PCR & Gel Band Purification Kit

Request Price for GRS PCR & Gel Band Purification Kit

FAQs

How to use GRS PCR & Gel Band Purification Kit:
PROTOCOL

1) Transfer 5µl of PCR product to a new tube
2) Add 1µl of rSAP and 1µl of Exo I
3) Incubate at 37ºC for 5 minutes
4) Heat-inactivate at 80ºC for 10 minutes

The purified PCR product can be used for DNA sequencing reaction immediately