The GRS PCR & Gel Band Purification Kit provides an efficient and fast method for the purification and or concentration of high quality DNA fragments (70bp to 15kb) from PCR reactions, enzymatic restriction digestion or from agarose gels. Eluted DNA is suitable for all common downstream applications including PCR, enzymatic restriction digestion, cloning, library construction, Southern blot analysis, and DNA sequencing.
The GRS PCR & Gel Band Purification Kit uses chaotropic salts to dissolve agarose gel and to denature enzymes such as restriction enzymes or polymerases. The buffer system is optimized to allow selective binding of DNA to the glass fiber matrix of the spin column (1). Contaminants such as proteins, divalent cations, unincorporated nucleotides, and enzyme inhibitors are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified genomic DNA is subsequently eluted by a low salt Elution Buffer or TE or water. The entire procedure can be completed in approximately 20 minutes without phenol/chloroform extraction or alcohol precipitation, with a typical DNA recovery of 80% to 90% for agarose gel extraction and up to 95% for PCR or Restriction Enzyme reactions.