GRiSP

GRS Protein Marker MultiColour PLUS

Ready-to-use three-colour protein standard suitable for monitoring protein separation during SDS-PAGE, size determination of proteins, and verification of transfer efficiency of Western Blotting onto membrane (compatible with PVDF, nylon and nitrocellulose). This marker is composed of 10 pre-stained proteins ranging from 6,5kDa to 270kDa. For easy identification of each band, it includes three reference bands of different colours that co-migrate with proteins of 30kDa (red), 52kDa (green) and 270kDa (red), respectively, when separated by 4-20% gradient SDS-PAGE using TGS Buffer.

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Description

Ready-to-use pre-stained protein marker suitable for monitoring protein separation during SDS-PAGE, verification of Western Transfer Efficiency onto membranes and for estimation of the molecular weight of proteins and/or polypeptides in the range of 10-250kDa. The GRS Protein Marker Multicolour is composed of 12 bands: 10 blue bands and 2 reference bands with different colours for easy identification (one red band (~25kDa) and one green band (~75kDa)).

 

Additional information

Quantidade

3x 500ul, 500ul

Features

Quantity: 500μl of approx. 0.1~0.4 mg/ml of each protein in 20mM Tris-phosphate , pH 7.5 (25°C), 2% SDS, 1mM Dithiothreitol, 3.6M Urea, and 15% (v/v) Glycerol.

Storage: Up to 2 weeks at room temperature, 3 months at +4ºC, 2 years at -20ºC.

Contents (kDa)
6.5 | 16 | 30 | 37 | 52 | 66 | 95 | 130 | 175 | 270 (Tris-Glycine 4-20%)

Recommended Loading
3μl or 5μl for clear visualization during electrophoresis on mini-gel (100-150 lanes).
1.5~2.5 μl per well for general Western transferring (200-300 lanes).
Apply more for thicker (> 1.5 mm) or larger gels.

Documents

Download Product Info Datasheet

Download MSDS GLP03 GRS Protein Marker MultiColour PLUS v73503

Photos

Order Information

Reference Description Quantity
GLP03.0500 GRS Protein Marker MultiColour Plus (100 lanes) 500uL
GLP03.3500 GRS Protein Marker MultiColour Plus (300 lanes) 3x 500uL

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Product: GRS Protein Marker MultiColour PLUS

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FAQs

How to use GRS Protein Marker MultiColour PLUS:
Quality Control
Functionally tested by 15% SDS-PAGE (using Tris-Glycine Buffer) followed by Western Blotting onto nitrocellulose membrane.