GRiSP

GRS Unstained Protein Marker

Ready-to-use unstained protein standard suitable for size determination of proteins in SDS-PAGE. This marker is composed of 8 unstained proteins and 1 pre-stained protein (blue) for monitoring of electrophoresis and verification of transfer efficienc

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Description

Ready-to-use unstained protein standard suitable for size determination of proteins in SDS-PAGE. This marker is composed of 8 unstained proteins and 1 pre-stained protein (blue) for monitoring of electrophoresis and verification of transfer efficiency of Western Blotting onto membrane (compatible with PVDF, nylon and nitrocellulose). This unstained proteins range from 20kDa to 120kDA, whereas the pre-stained protein co-migrates with proteins of approximately 12kDa (depending on the SDS-PAGE conditions). For easy identification of each band, the 50kDa band has double intensity to serve as an internal reference.

 

Additional information

Quantidade

1x 250 ul, 2x 250ul, 6x 250 uL

Features

Quantity: 1x or 2x 250μl (see order information) of approx. 0.1 mg/ml of each unstained protein (0.2 mg/ml for the 50kDa reference protein) and 0.4mg/ml of the stained reference band in 50mM Tris-phosphate, pH 6.8 (25°C), 5mM EDTA, 1% SDS, 10mM dithiothreitol, 0.01% phenol red, and 10% (v/v) glycerol.

Storage: -20ºC up to 2 years.

Contents (kDa)
12** | 20 | 30 | 40 | 50 | 60 | 80 |100 | 120 (Tris-Glycine 12%)
**)The apparent molecular weight of pre-stained proteins depends on the electrophoretic conditions and has been determined for SDS-PAGE using Tris-Glycine (12%).

Recommended Loading
3μl or 5μl for electrophoresis on mini-gel (100-150 lanes).
Apply more for thicker (> 1.5 mm) or larger gels.

Ready to use
The protein marker is ready to use. There is no need for heating and/or adding of reducing agent.

Documents

Download Product Info -GRS Unstained Protein Marker 250ul

Download Product Info – GRS Unstained Protein Marker 500ul

Download MSDS GLP10 GRS Unstained Protein Marker v73503

Order Information

Reference Description Quantity
GLP10.0500 GRS Unstained Marker 2x 250ul
GLP10.3500 GRS Unstained Marker 6x 250ul
GLP10.0250 GRS Unstained Marker 1x 250ul

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Product: GRS Unstained Protein Marker

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FAQs

How to use GRS Unstained Protein Marker:
Quality Control
Functionally tested by 12% SDS-PAGE (using Tris-Glycine Buffer) followed by Coomassie Brilliant Blue Staining and/or Western Blotting onto nitrocellulose membrane.