Description
The GRS Pure DNA Kit provides an efficient and fast method for the purification and or concentration of high quality DNA (50bp to 30kb) from samples containing partial purified DNA (genomic DNA, mitochondrial DNA, PCR products, etc) obtained via other DNA isolation methods. The purified DNA is suitable for all common downstream applications including PCR, RFLP, cloning, library construction, Southern blot analysis, and DNA sequencing.
Principle
The GRS Pure DNA Kit uses a unique DNA Binding Buffer that is optimized to allow easy binding of DNA to the glass fiber matrix of the spin column (1). Contaminants such as proteins, divalent cations, unincorporated nucleotides, and enzyme inhibitors are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified DNA is subsequently eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in approximately 20 minutes without phenol/chloroform extraction or alcohol precipitation, with a typical DNA recovery of 80% to 90%.