Description
The GRS Total RNA Kit – Tissue – provides an efficient and fast method for the purification and or concentration of high quality total RNA (including mRNA, tRNA and rRNA) from a variety of animal and paraffin-embedded tissues. Optional DNAse treatment can be included in the protocol to remove undesired DNA residue. Eluted purified RNA is suitable for RT-PCR, Northern Blotting, mRNA selection, cDNA synthesis, and primer extension.
Principle
The GRS Total RNA Kit – Tissue – uses detergents and chaotropic salts to lyse cells and to denature proteins/inactivate RNAse. (If desired, an optional DNAse treatment step can be followed in order to remove unwanted residual DNA). The buffer system is optimized to allow selective binding of RNA to the glass fiber matrix of the spin column (1). Contaminants are completely removed using a Wash
Buffer (containing ethanol) in a simple centrifugation step. The purified RNA is subsequently eluted with RNAse-Free Water. The entire procedure can be completed within 60 minutes with typical RNA yields of 5-30μg.