Ponceau S Solution

Ponceau S Solution

Ponceau S Solution allows for the rapid and reversible detection of proteins on nitrocellulose and PVDF membranes for the verification of the transfer efficiency of Western Blotting before proceeding with incubation with primary antibody.

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Description

Ponceau S Solution allows for the rapid and reversible detection of proteins on nitrocellulose and PVDF membranes for the verification of the transfer efficiency of Western Blotting before proceeding with incubation with primary antibody. Transfer artifacts, such as uneven transfer or bubbles, can be quickly detected, preventing continuation with imperfect membranes, saving you time and expensive antibodies. Ponceau S does not damage the antigen or has any deleterious effects on the sequence of blotted proteins and polypeptides and is therefore the method of choice for location for blot?sequencing. Staining can be carried out in less than 5 minutes and destaining also takes only a few minutes of incubation in water. After destaining, one can proceed immediately with subsequent steps of the Western Blotting protocol.

 

Features

Product: ready-to-use Ponceau S solution for the reversible staining of proteins blotted onto nitrocellulose or PVDF membranes.
Quantity: 500ml
Storage: 2ºC – 25ºC for at least 1 year.

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Product Info

Protocols

1. After Western Blotting, image the blot for permanent record, and then rinse the blot with 20ml of water for 5 minutes at room temperature, using an orbital shaker.
2. Decant and incubate the membrane in a container containing 10-20ml of GRS Stripping Buffer (membrane must be completely covered) at room temperature for 5-10 minutes, using an orbital shaker
3. After stripping, wash the membrane extensively (min 3x) with wash buffer (e.g. TBS(T) or PBS(T)).
4. After washing, block the membrane as usual.
5. After blocking the membrane is ready for the next detection with other set of antibodies.

Note: Because Stripping may reduce signal intensity, it is recommended to probe first for the antigen with the lowest level of expression. If more stringent stripping is required, one could add reducing agent (DTT or β-mercaptoethanol) to the stripping buffer and/or heat the blot during the stripping procedure.

Order Information

GB21.0500 Ponceau S Solution 500mL