Ponceau S Solution allows for the rapid and reversible detection of proteins on nitrocellulose and PVDF membranes for the verification of the transfer efficiency of Western Blotting before proceeding with incubation with primary antibody. Transfer artifacts, such as uneven transfer or bubbles, can be quickly detected, preventing continuation with imperfect membranes, saving you time and expensive antibodies. Ponceau S does not damage the antigen or has any deleterious effects on the sequence of blotted proteins and polypeptides and is therefore the method of choice for location for blot?sequencing. Staining can be carried out in less than 5 minutes and destaining also takes only a few minutes of incubation in water. After destaining, one can proceed immediately with subsequent steps of the Western Blotting protocol.