RNase Inhibitor (40 U/uL)

GRiSP´s RNase Inhibitor specifically inhibits common RNases, including RNase A, RNase B, and RNase C.

 

SKU N/A Category

Description

Trace amounts of ribonucleases (RNases) can be introduced into RNA preparations via contaminated plasticware or reagents, or by co-purification with the isolated RNA. Presence of tiny amounts of contaminating (RNases) may compromise many downstream applications such as expression analysis. GRiSP´s RNase Inhibitor specifically inhibits common RNases, including RNase A, RNase B, and RNase C. As the RNase inhibitor does not interfere with DNA polymerase activity, it is an extremely useful additive in PCR and RT-PCR. GRiSP´s RNase Inhibitor is a sturdy enzyme with improved resistance to oxidation, being stable under very low concentrations (<1mM) of DTT. It is therefore an excellent choice for the protection of your valuable RNA samples.

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Product Info 

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Protocols

In a typical cDNA synthesis reaction mixture set-up, we recommend adding RNase Inhibitor, just prior to adding reverse transcriptase, to a final concentration of 1U/µl (e.g. 0.5µl in a total of 20µl reaction volume).

Order Information

GE85.0100 RNase Inhibitor (40 U/uL) 100uL