GRiSP

SGTB Agarose Electrophoresis Buffer

SGTB is a NEW buffer ideal for Agarose Electrophoresis of DNA fragments of 100bp to 1000bp

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Description

Time Saving
SGTB allows much FASTER RUNS
Unlike TAE and TBE gels, which tolerate only about 110V, the low-conductance electrophoretic buffer SGTB, allows for the application of high voltage 200-300V without generating a lot of heat, so your gel won´t melt.

High Resolution
DNA agarose gel electrophoresis using SGTB results in very sharp bands. Faster Runs result in less fuzzy bands.

Better Separation
Using SGTB results in larger differences in relative mobility (Rf), especially between smaller DNA fragments.

Stronger and Clearer
Gels made with SGTB are also CLEARER, increasing detection sensitivity; and STRONGER, making them excelent for blotting.

IF you already get the separation you need with TBE/TAE, you can get the same resolution with SGTB using less agarose. With SGTB you save TIME and MONEY!

Additional information

Quantidade

1L 20X, 5L 10X, 5L 20X

Documents

Download Product info Datasheet

Download MSDS GB01 SGTB (20X) v7E502

Order Information

Reference Description Quantity
GB01.0120 SGTB Agarose Electrophoresis Buffer 1L 20X
GB01.0520 SGTB Agarose Electrophoresis Buffer 5L 20X

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Product: SGTB Agarose Electrophoresis Buffer

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FAQs

How to use SGTB Agarose Electrophoresis Buffer:
Prepare 1x SGTB DNA electrophoresis buffer by diluting the concentrate in pure water | Prepare the agarose gel with 1x SGTB, as usual | Fill the electrophoresis chambers with 1x SGTB and put the gel casting tray into place (the first time, chambers need to be washed with 1x SGTB in order to remove residual previous buffer) | For optimal resolution, cover the gel with 3-5 mm of SGTB | Apply samples and run the gel at constant voltage, using a higher voltage than usual (GRiSP suggests doubling the voltage you normally use and optimize gradually) | The optimal voltage depends, among other factors, on the type and percentage of agarose, as well as on the nature of the sample. Therefore, occasionally the optimal voltage might be less than 2x the normal voltage | The run time will be reduced considerably!