Description
Dehydrated powder for the preparation of nutritionally rich liquid medium for the preparation and transformation of Escherichia coli.
Selected:
Dehydrated powder for the preparation of nutritionally rich liquid medium for the preparation and transformation of Escherichia coli.
Quantity: 500g
Appearance: Beige powder. Autoclaved medium should be amber, slightly opalescent.
Storage: 2ºC – 25ºC. When not in use, keep container closed to avoid hydration.
Formulation (g/L)
Tryptone: 20,00
Yeast Extract: 5,00
NaCl: 0,50
MgSO4: 0,96
KCl: 0,186
Final pH (25ºC): 7,0 ± 0,2
Preparation:
Add 26,6g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121ºC for 15 to 20 minutes. Store at 2ºC to 8ºC.
Supplements
SOB medium is a rich growth medium, optimized for the preparation and transformation of competent cells. Transformation requires perforation of bacteria in order to allow DNA to enter the cell. In order to help to survive this process, competent cells need isotonic rich media such as SOB. It contains all the nutritional requirements for E.coli. Tryptone and Yeast Extract are the sources for carbon, nitrogen, vitamins, minerals, and amino acids essential for growth. Sodium chloride and Potassium chloride supply essential electrolytes for transport and osmotic balance, whereas Magnesium sulfate serves as a source for magnesium ions.
GCM12.0500 | SOC Medium | 500g |
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