Description
Dehydrated powder for the preparation of LB Broth w/o trace elements, supplemented with glucose and alpha lactose for the auto induction of protein expression under the control of IPTG-inducible promoters in Escherichia coli.
Terrific Broth (Auto Induction Medium)
Dehydrated powder for the preparation of Super Broth w/o trace elements, supplemented with glucose and alpha lactose for the auto induction of protein expression under the control of IPTG-inducible promoters in Escherichia coli.
Features
Quantity: 500g
Appearance: Beige powder. Autoclaved medium should be amber.
Storage: 2ºC – 25ºC. When not in use, keep container closed to avoid hydration.
Preparation:
Add 55,55g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121ºC for 15 to 20 minutes. Store at 2ºC to 8ºC.
Formulation (g/L)
Tryptone: 12,00
Yeast Extract: 24,00
MgSO4: 0,15
(NH4) 2SO4: 3,30
KH2PO4: 6,50
Na2HPO4: 7,10
Glucose 0,50
Alpha Lactose 2,00
Final pH (25ºC): 7,0 ± 0,2
Order Information
| GCM19.0500 | Terrific Broth (AIM) | 500g |