Description
The TripleXtractor directRNA kit combines the strong lysis capability of the monophasic phenol/guanidine thiocyanate solution “TripleXtractor Direct” with a spin column system for convenient, rapid and cost effective purification of ultrapure RNA without the need of chloroform phase separation and isopropanol precipitation.
Principle
Following a one-step homogenization/lysis step with TripleXtractor Direct, in which cells are disrupted and cellular components are dissolved efficiently, while maintaining RNA integrity, the sample is passed through a RNA binding spin column. Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. Optional DNAse treatment can be included
in the protocol. The purified RNA is subsequently eluted by RNAse-free water. The entire procedure can be completed in approximately 15 minutes with a typical A260/A280>1,9 and A260/A230 >1,9. The purified total RNA is free of DNA and proteins and can be used for all common downstream applications such as cDNA Library construction, Nothern Blotting, RT-PCR, in vitro translation, Nuclease Protection Assays, etc.