GRiSP

WesternBright ECL HRP substrate

The best choice for routine, film-imaged Western blots!

 

Clear

Description

The best choice for routine, film-imaged Western blots

WesternBright ECL is more sensitive than other chemiluminescent substrates, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. Save time and money with WesternBright ECL.

 

Additional information

Quantidade

200mL, 500mL

Features

  • Stronger signal than Amersham™ ECL or Pierce™ ECL
  • Detect low pg protein amounts
  • Use up to ten-times less antibody

WesternBright ECL is a horseradish peroxidase substrate optimized for chemiluminescent Western blots imaged using X-ray film. WesternBright ECL requires as little as ten times less antibody than other substrates, allowing you to save precious antibodies and samples. Additionally, the WesternBright ECL signal is long lasting, allowing multiple exposures to be carried out without substantial signal decay.

ECL Figure 1
WesternBright ECL detects equal amounts of protein with up to ten times less antibody. Duplicate slot blots containing serial dilutions of transferrin were probed with the antibody dilutions shown, and detected with either WesternBright ECL or Pierce ECL (Thermo Scientific) according to the manufacturers’ instructions. The blots were imaged simultaneously on the same piece of film. WesternBright ECL produced the same signal with ten times less primary and five times less secondary antibody.

Documents

Advansta WB Guide

WesternBright ECL Info

WesternBright ECL Protocol

WesternBright ECL Faq

WesternBright ECL ApplicationNote

Photos

Video

Order Information

Reference Description Quantity
K-12045.D20 Western Bright ECL Kit 200ml
K-12045-D50 Western Bright ECL Kit 500ml

Ask a Question

Note that by submitting this contact form, you are providing GRISP with some personal information. We encourage you to take the time to review our revised Privacy Policy.

Product: WesternBright ECL HRP substrate

Request Price for WesternBright ECL HRP substrate

FAQs

How to use WesternBright ECL HRP substrate:
Ver docs Abaixo