WesternBright ECL HRP substrate

The best choice for routine, film-imaged Western blots!


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The best choice for routine, film-imaged Western blots

WesternBright ECL is more sensitive than other chemiluminescent substrates, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. Save time and money with WesternBright ECL.



  • Stronger signal than Amersham™ ECL or Pierce™ ECL
  • Detect low pg protein amounts
  • Use up to ten-times less antibody

WesternBright ECL is a horseradish peroxidase substrate optimized for chemiluminescent Western blots imaged using X-ray film. WesternBright ECL requires as little as ten times less antibody than other substrates, allowing you to save precious antibodies and samples. Additionally, the WesternBright ECL signal is long lasting, allowing multiple exposures to be carried out without substantial signal decay.

ECL Figure 1
WesternBright ECL detects equal amounts of protein with up to ten times less antibody. Duplicate slot blots containing serial dilutions of transferrin were probed with the antibody dilutions shown, and detected with either WesternBright ECL or Pierce ECL (Thermo Scientific) according to the manufacturers’ instructions. The blots were imaged simultaneously on the same piece of film. WesternBright ECL produced the same signal with ten times less primary and five times less secondary antibody.


Advansta WB Guide

WesternBright ECL Info

WesternBright ECL Protocol

WesternBright ECL Faq

WesternBright ECL ApplicationNote



Order Information

K-12045-D20 WesternBright ECL- HRP Substrate for X-ray Film 200ml
K-12045-D50 WesternBright ECL- HRP Substrate for X-ray Film 500ml