Xpert AmpliFi Hotstart DNA Polymerase is an all-round and robust high-fidelity enzyme with enhanced DNA binding, resulting in improved processivity, shorter extension times, higher yield and capacity to amplify more difficult and longer targets. Combined with its extremely low error-rate and all the advantages of a new proprietary reversible hotstart technology, this makes Xpert AmpliFi Hotstart ideal for applications such as high-fidelity PCR, site-directed mutagenesis, multiplexing, long-range PCR and NGS sequencing, when robustness, specificity, sensitivity and proof-reading are extremely important.
Xpert AmpliFi Hotstart DNA polymerase has been derived from Pfu DNA polymerase for its proof-reading activity. Xpert AmpliFi has a very high accuracy resulting in fidelity that is about 100x higher than regular Taq DNA polymerase. Hotstart is achieved using a proprietary aptamer-like molecule that reversibly inhibits both 5´-3´polymerase activity and 3´-5´exonuclease activity. It prevents the formation of primer-dimers and non-specific amplification, increasing the sensitivity and specificity of the PCR reaction. Moreover, this allows preparing reaction mixtures at room temperature with pre- and post-PCR stability for up to 24h.