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Xpert AmpliFi Hotstart

Xpert AmpliFi Hotstart DNA Polymerase is an all-round and robust high-fidelity enzyme with enhanced DNA binding, resulting in improved processivity, shorter extension times, higher yield and capacity to amplify more difficult and longer targets. Combined with its extremely low error-rate and all the advantages of a new proprietary reversible hotstart technology, this makes Xpert AmpliFi Hotstart ideal for applications such as high-fidelity PCR, site-directed mutagenesis, multiplexing, long-range PCR and NGS sequencing, when robustness, specificity, sensitivity and proof-reading are extremely important.

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Description

Xpert AmpliFi Hotstart DNA Polymerase is an all-round and robust high-fidelity enzyme with enhanced DNA binding, resulting in improved processivity, shorter extension times, higher yield and capacity to amplify more difficult and longer targets. Combined with its extremely low error-rate and all the advantages of a new proprietary reversible hotstart technology, this makes Xpert AmpliFi Hotstart ideal for applications such as high-fidelity PCR, site-directed mutagenesis, multiplexing, long-range PCR and NGS sequencing, when robustness, specificity, sensitivity and proof-reading are extremely important.

Xpert AmpliFi Hotstart DNA polymerase has been derived from Pfu DNA polymerase for its proof-reading activity. Xpert AmpliFi has a very high accuracy resulting in fidelity that is about 100x higher than regular Taq DNA polymerase. Hotstart is achieved using a proprietary aptamer-like molecule that reversibly inhibits both 5´-3´polymerase activity and 3´-5´exonuclease activity. It prevents the formation of primer-dimers and non-specific amplification, increasing the sensitivity and specificity of the PCR reaction. Moreover, this allows preparing reaction mixtures at room temperature with pre- and post-PCR stability for up to 24h.

Additional information

Quantidade

1.25mL, 100U, 5x 1.25mL

Features

Properties:
Amplicon size:  up to 20kb
Extension Rate: 2-6kb/min
Hotstart:           Yes
A-overhang:      No
Error-rate:  100x lower than Taq

Applications:
– High-fidelity PCR
– Complex targets (including GC-rich and AT-rich templates)
– Longe-Range PCR
– site-directed mutagenesis
– NGS
– Multiplex

Components:
– Kit version with dNTPs in separated buffer or
– Mastermix

Storage:
-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.

Documents

Product Info Xpert AmpliFi Hotstart (Kit)

Product Info Xpert AmpliFi Hotstart MasterMix

Order Information

Reference Description Quantity
GE37.0100 Xpert AmpliFi Hotstart (kit with dNTPs included) 100U
GE47.0100 Xpert AmpliFi Hotstart MasterMix 1.25ml
GE47.5100 Xpert AmpliFi Hotstart MasterMix 5x 1.25ml

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FAQs

How to use Xpert AmpliFi Hotstart:
Functionally tested in PCR. Bacterial DNA free (tested by PCR). Absence of endonucleases, exonucleases, and ribonucleases was confirmed by appropriate assays.